Cryopreservation of gametes and embryos of salmonid fishes

Risk of Contamination of Gametes and Embryos during Cryopreservation and Measures to Prevent Cross-Contamination

The introduction and widespread application of vitrification are one of the most important achievements in human assisted reproduction techniques (ART) of the past decade despite controversy and unclarified issues, mostly related to concerns about disease transmission. Guidance documents published by US Food and Drug Administration, which focused on the safety of tissue/organ donations during Z...

Cryopreservation of Porcine Gametes, Embryos and Genital Tissues: State of the Art

I-11: Cryopreservation of Canine Embryos

Background: The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer and cryopreservation of gametes have considerably contributed to the development of biomedical sciences in addition to improved breeding in domestic animals and infertility treatment in humans. However, ARTs used in canine species have strictly limited utility when compared with other mammali...

Obtaining gametes and embryos of ascidians.

Ascidians are basal chordates that have become increasingly important for understanding chordate evolution. They comprise three orders. In the orders Phlebobranchia and Stolidobranchia, most species freely spawn eggs and sperm, whereas members of the order Aplousobranchia form colonies that brood their eggs and broadcast sperm. In the two free spawning orders, eggs and sperm are easily obtained...

Salmonid fishes undergoing the process of smoltification

have elevated levels of thyroid hormones (Barron, 1986; Hoar, 1988; Datta et al., 1999; Eales, 1990). Thyroid hormones play a central role in controlling many of the alterations in physiology, morphology and behavior noted during this metamorphic transition in juvenile salmon (Hoar, 1988). Thyroid hormones have also been identified in altering the visual system during smoltification. During mig...